Supplied as a premixed solution that can be used like an ethidium bromide solution. Egel ibase power system safe imager realtime transilluminator. As per manufacturers instructions invitrogen, sybr safe dna gel stain was added. Visualization of detection nucleic acid is an evolving and rapidly growing discipline based on big emerging field.
Sybr safe dna gel stain was evaluated in a battery of toxicity and mutagenicity tests and found to be a safer alternative to ethidium bromide. Exposure controlspersonal protection prevent further leakage or spillage if safe to do so. Egel agarose gels with sybr safe thermo fisher scientific. Development of a sybr safe technique for the sensitive.
White paper sybr safe dna gel stain sybr safe dna gel. The detection sensitivity of sybr safe dna gel stain is. This fast, easy staining protocol incorporates sybr safe into the molten agarose before the gel is poured into the casting tray. Sybr safe stain comes either as a concentrate or as a readytouse solution that can. Bound to nucleic acids, sybr safe stain has fluorescence excitation maxima at 280 and 502. Sybr is a safer stain for dna than ethidium bromide. It has been repeatedly argued that the sybr green i method results in more precise and sensitive assays 4,8,12. The main advantage sybr safe has over ethidium bromide and the other sybr dyes is that sybr safe can go down the sink or in the trash can without getting your lab written up for polluting. Sybr green pcr master mix and rtpcr reagents protocol. To request the quick reference card qrc or for additional information, contact.
Nov 01, 2005 sybr safe photographic filter the sybr safe photographic filter s37100, photographic filters for fluorescent dyestained gels and blots is ideal for blackandwhite photography of gels stained with the sybr safe dna gel stain section 8. Bound to nucleic acids, sybr safe stain has fluorescence. You can put multiple gels in a bath, just need to make enough to cover the gels. Ethidium bromide, propidium iodide, acridine orange, sybr green i, sybr green ii, sybr gold, gelstar. These dyes have been determined to have mutagenic properties. Sybr safe is not classified as hazardous waste under the resource conservation and recovery act rcra and is not a regulated waste under the commonwealth of massachusetts hazardous waste regulations. Sybr safe sybr safe is an intercalator for nucleic acid and is propriety of life science technology. Safer than ethidium bromide sybr safe dna gel stain has been specifically formulated and evaluated in a battery of toxicity tests with results indicating that it is less mutagenic and safer than ethidium bromide. The upfront cost of sybr safe is initially more expensive than ethidium bromide, as table 1 illustrates.
Sybr safe dna gel stain is a highly sensitive stain for visualization of dna in agarose or acrylamide gels. I have read about an alternative stain sybr green ii, but if i want to use sybr safe, i have heard of modifications such as. Although one of the most popular dyes which are used for the most common molecular biology application is the ethidium bromide. Sybr safe, 10,000x in dmso thermo fisher scientific s33102. The 1 l unit size s33100 provides sufficient material to stain 20 minigels. The agarose sybr safe stain mixture may be heated in the microwave. Sybr safe has to be mixed 110,000 in the same buffer in which you made your gel and the gel can be soaked in it before or after it is run.
For a single small gel, can use a small tuppy and make 200mls by adding 20l of sybr safe to 200mls of 1x faster better buffer. Comparison of nucleic acid gel stains cell permeability. The invitrogen logo should be located at the bottom. Sybr safe dna gel stain from thermo fisher scientific. Sybr safe is a fluorescent dna gel stain developed at molecular probes that exhibits sensitivity similar to that of ethidium bromide, while being considerably safer and environmentally friendly. Need for protection from light during gel staining not necessary recommended recommended not necessary storage temperature room. Sybr gold nucleic acid gel stain none none none none none section 8.
A highthroughput dna extraction system suitable for conifers. Sensitivity evaluation of sybr green i, sybr safe and. Sybr safe dna gel stain thermo fisher scientific us. Sybr safe dna gel stain is nontoxic, noncarcinogenic, and. Five ultracentrifugation tubes were set up, ultracentrifuged and visualized as described previously for sybr.
Invitrogen sybr safe dna gel stain in 1x tae 1l invitrogen. Sybr safe dna gel stain is a better nucleic acid staining reagent for all of your molecular biology needs. Instructions are provided below for using egel precast agarose gels with sybr safe dna gel stain in the egel powerbase v. Sybr safe stain is specifically formulated to be a less hazardous alternative to ethidium bromide that can be used with either bluelight or uv excitation. Five ultracentrifugation tubes were set up, ultracentrifuged and visualized as described previously for sybr safe, each one containing 2000ng of 12 cdna and either 0, 100, 200, 500, or 2000ng of cdna from m. In aquatic toxicity tests in fathead minnows ca title 22 acute screening methodology, sybr safe dna gel stain in 0. Thus, 254 nm or 300 nm uvexcitation will work, as will 488 nm lasers, 470 nm leds, and broad blue excitation such as invitrogen s safe imager coming september 2005 and clare chemicals dark reader. Thus, 254 nm or 300 nm uvexcitation will work, as will 488 nm lasers, 470 nm leds, and broad. When bound to nucleic acids, the greenfluorescent sybr safe stain has fluorescence excitation maxima at 280 and 502nm, and an emission maximum at 530nm.
Bound to nucleic acids, the greenfluorescent sybr safe stain has fluorescence excitation maxima at. Sybr gold nucleic acid gel stain thermo fisher scientific. Sensitivity evaluation of sybr green i, sybr safe and calcein. Sybr safe dna gel stain was weakly mutagenic only with. Sybr safe is a fluorescent dna gel stain developed at. Egel with sybr safe gels combine the convenience of the bufferless egel precast agarose. For more information and detailed instructions, refer to. The detection limit of dna in cscl gradients prepared with sybr safe as a dye was determined. Sybr safe stain is specifically formulated to be a less hazardous alternative to ethidium bromide eb that can be used with either bluelight or uv excitation. Identification of the substancemixture and of the companyundertaking identification of the substance or mixture product code s33102 product name.
The egel safe imager realtime transilluminator is designed for viewing egel with sybr safe gels and egel clonewell gels on the laboratory benchtop for realtime monitoring on the egel ibase power system or for documentation purposes at the end of the run directly on the egel safe imager transilluminator. If using the 10,000x sybr safe stain concentrate, dilute the concentrated stain 1. Chemical namesweden occupational exposure limits tlvs llvs netherlands oel macspain oel twa united kingdom personal precautions dimethyl sulfoxide 67685. Sybr safe is a cyanine dye used as a nucleic acid stain in molecular biology. Sybr safe dna gel stain has been specifically developed for reduced mutagenicity, making it safer than ethidium bromide for staining dna in agarose or acrylamide gels.
What is the use or function of sybr safe dna gel stain. Excellent sensitivity sybr safe stain offers excellent sensitivity in nucleic acid visualization and documentation applications with either uv excitation or bluelight excitation. Sybr safe is an intercalator for nucleic acid and is propriety. Sybr safe stain is specifically formulated to be a less hazardous alternative to ethidium bromide that can utilize either blue light or uv excitation. Supplied as a premixed solution that can be used like an ethidium bromide solution, either in the gel during the electrophoresis run or as a postrun stain. Sybr safe stain is supplied as a concentrate solution that can be used like an ethidium bromide solution.
Also, sybr safe can go all over your bare skin without you having to file an incident report. Sybr safe dna gel stain is a highly sensitive stain for visualization of dna or rna in agarose or acrylamide gels. Easy to use sybr safe stain is supplied as a 10,000x concentrate in dmso which can be used like a solution of ethidium bromide. Sybr safe stain can also be mixed into an agarose gel for staining during electrophoresis or the gel can be incubated in a solution of sybr safe stain following electrophoresis. Bluelight transilluminators, such as invitrogen s safe imager bluelight transilluminator also show excellent sensitivity with sybr gold stained gels. Sybr safe stain is supplied as either a concentrate or a readytouse. Invitrogen sybr safe dna gel stain is a highly sensitive stain for visualization of dna in agarose or acrylamide gels. Sybr safe dna gel stain is nontoxic, noncarcinogenic, and classified as nonhazardous waste. Bound to nucleic acids, the greenfluorescent sybr safe stain has fluorescence excitation maxima at 280 and 502nm, and an emission maximum at 530nm.
Technical guide contains information about egel precast agarose gels and is intended to supplement the quick reference cards supplied with egel products. Sybr safe stain is less mutagenic than ethidium bromide and offers better sensitivity than ethidium bromide. The sybr green ibased quantification method is the method of choice for these applications. Sybr safe is one of a number of sybr dyes made by the life technologies corporation. Sybr safe dna gel stain is supplied readytouse in two sizes. Sybr safe stain is specifically formulated to be a. All gels that have been cast with these dyes in them, unwanted dye stock solutions, and all contaminated debris must be collected for disposal by the hwmu. Sybr safe photographic filter the sybr safe photographic filter s37100, photographic filters for fluorescent dyestained gels and blots is ideal for blackandwhite photography of gels. I would like to know any experience on use of sybr safe to stain rna. White paper sybr safe dna gel stain sybr safe dna gel stain. Invitrogen sybr safe dna gel stain fisher scientific. It contains antibodymediated hotstart itaq dna polymerase, dntps, mgcl 2, sybr green i dye, enhancers, stabilizers, and a blend of. The electrophoretic analysis was analysed for further confirmation of the naked eye visualization and the turbidity of magnesium phyrophosphate were recorded using.
Life technologies corporation andor its affiliates warrant their. Stained gels may also be viewed with 300 nm ultraviolet or 254 nm epi or transillumina. Molecular probes sybr green i nucleic acid gel stain is one of the most sensitive stains available for detecting doublestranded dna dsdna in agarose and polyacrylamide gels. Search results for sybr safe dna gel stain at sigmaaldrich. And store at 20c for longterm storage in vitro stock solutions soluble in dmso preparing mass solvent 1mg 5mg 10mg concentration 1 mm 1. With 300 nm transillumination, as little as 60 pg dsdna per band can be detected with sybr green i stain. Sybr safe dna gel stain is not only better for you and the environment, but also your sample and your institution. Egel with sybr safe gels combine the convenience of the bufferless egel precast agarose gels with the safety of sybr safe dna gel stain. For a single small gel, can use a small tuppy and make 200mls by adding. It is marketed as being less harmful than ethidium bromide, but this is debatable.
Sybr safe stain comes as a readytouse solution that can be used just like an ethidium bromide solution, and the detection sensitivity with. Details for sample preparation and electrophoresis conditions are included in this guide. An independent laboratory showed reduced mutagenicity of sybr safe stain compared to ethidium bromide using the ames test. The mix is optimized for sybr green re actions and contains sybr. Sybr safe stain is specifically formulated to be a less hazardous alternative to.
The stain is also suitable for staining rna in gels. Sybr safe stain comes as a premixed solution that can be used just like an ethidium bromide solution, either in the gel during the electrophoresis run or as a postrun stain. In aquatic toxicity tests in fathead minnows ca title 22 acute screening. Fact sheet green features product description sybr safe dna gel stain is offered as either a concentrate or a readytouse solution. Electrophoresis how does products such as sybr safe and. A better dna stain sybr safe dna gel stain is a better nucleic acid staining reagent for molecular biology applications. Egel agarose gels with sybr safe fisher scientific. This means that using sybr safe dna gel stain not only helps to reduce exposure to. Identification of the substancemixture and of the companyundertaking identification of the substance or mixture product code s33102 product name sybr safe dna gel stain companyundertaking identification 24 hour emergency response for hazardous materials or dangerous goods incident.
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